HEp-2 人喉表皮樣癌細(xì)胞
ATCC® Number: CCL-23?
Additional information about this cell line
Designations: HEp-2
Depositors: AE Moore
Biosafety Level: 2 [Cells contain Papovavirus ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: epithelial
Source: Organ: HeLa contaminant
Cellular Products: keratin
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Virus Susceptibility: Human adenovirus 3
Human poliovirus 1
Vesicular stomatitis virus
Reverse Transcript: negative
DNA Profile (STR): Amelogenin: X
CSF1PO: 9,10
D13S317: 12,13.3
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18
Cytogenetic Analysis: Occasional polyploids. Several marker chromosomes were observed along with frequent minutes, and often 2 large chromosomes with subterminal centromeres.HeLa Marker Chromosomes: One copy of M2, two-four copies of M3 and one copy of M4 as revealed by G-banding patterns.
Isoenzymes: G6PD, A
HeLa Markers: Y
Comments: Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination. This line was originally thought to be derived from an epidermoid carcinoma of the larynx, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination. The cells are positive for keratin by immunoperoxidase staining.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.053 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: culture medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 22149: Moore AE, et al. Culture characteristics of four permanent lines of human cancer cells. Cancer Res. 15: 598-602, 1955. PubMed: 13261081
22263: Chen TR. Re-evaluation of HeLa, HeLa S3, and HEp-2 karyotypes. Cytogenet. Cell Genet. 48: 19-24, 1988. PubMed: 3180844
25970: Toolan HW. Transplantable human neoplasms maintained in cortisone-treated laboratory animals: H.S. No. 1; H.Ep. No. 1; H.Ep. No. 2; H.Ep. No. 3; and H.Emb.Rh. No. 1. Cancer Res. 14: 660-666, 1954. PubMed: 13209540
26127: Black FL, et al. Propagation of measles virus in a strain of human epidermoid cancer cells (Hep-2). Proc. Soc. Exp. Biol. Med. 93: 107-108, 1956. PubMed: 13370591
26129: . . Tex. Rep. Biol. Med. 15: 588, 1957.
26130: Moore AE. Tumorigenic activity of cultures. Ann. N.Y. Acad. Sci. 76: 497-505, 1958. PubMed: 13627875
32299: St. Geme JW, et al. Characterization of the genetic locus encoding Haemophilus influenzae type b surface fibrils. J. Bacteriol. 178: 6281-6287, 1996. PubMed: 8892830
32469: Gromeier M, et al. Internal ribosomal entry site substitution eliminates neurovirulence in intergeneric poliovirus recombinants. Proc. Natl. Acad. Sci. USA 93: 2370-2375, 1996. PubMed: 8637880
32519: Roller RJ, et al. Structure and function in the herpes simplex virus 1 RNA-binding protein US11: mapping of the domain required for ribosomal and nucleolar association and RNA binding in vitro. J. Virol. 70: 2842-2851, 1996. PubMed: 8627758
