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產(chǎn)品名稱:
小鼠血液細(xì)胞
產(chǎn)品型號(hào):
TIB-68
產(chǎn)品廠商:
美國標(biāo)準(zhǔn)生物品收藏中心（ATCC）
產(chǎn)品文檔:
無相關(guān)文檔
簡單介紹
TIB-68 WEHI-3 小鼠血液細(xì)胞,原代細(xì)胞、細(xì)胞系。細(xì)胞庫管理規(guī)范，提供的細(xì)胞株背景清楚，提供參考文獻(xiàn)和培養(yǎng)條件，
小鼠血液細(xì)胞
的詳細(xì)介紹
TIB-68 WEHI-3 小鼠血液細(xì)胞
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TIB-68?
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$303.00
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WEHI-3
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M Cohn
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Biosafety Level:
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1
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frozen
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See Propagation
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suspension (some adherent cells)
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Mus musculus (mouse)
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Organ: peripheral blood
Strain: BALB/c
Disease: leukemia
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lysozyme; granulocyte colony stimulating activity (CSA); interleukin-3 (interleukin 3, IL-3)
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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complement (C3)
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ATCC complete growth medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
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Medium Renewal: Every 2 to 3 days
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.
Maintain cell density between 2 X 10 exp5 and 2 X 10 exp6 viable cells/ml.
Adherent cells may be harvested by scraping.
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culture medium 95%; DMSO, 5%
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Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
recommended serum:ATCC 30-2020
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1080: Ralph P, et al. Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines. J. Exp. Med. 143: 1528-1533, 1976. PubMed: 1083890
1135: Ralph P, Nakoinz I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977. PubMed: 894031
1136: Ralph P, Nakoinz I. Direct toxic effects of immunopotentiators on monocytic myelomonocytic, and histiocytic or macrophage tumor cells in culture. Cancer Res. 37: 546-550, 1977. PubMed: 318922
23431: McKean DJ, et al. Major histocompatibility complex-restricted antigen presentation to antigen-reactive T cells by B lymphocyte tumor cells. J. Exp. Med. 154: 1419-1431, 1981. PubMed: 6170720
33035: Boring L, et al. Molecular cloning and functional expression of murine JE (monocyte chemoattractant protein 1) and murine macrophage inflammatory protein 1alpha receptors. J. Biol. Chem. 271: 7551-7558, 1996. PubMed: 8631787
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