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CCL-120.1 CCRF HSB-2 人急性**母細胞型白血病細胞

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產品名稱: CCL-120.1 CCRF HSB-2 人急性**母細胞型白血病細胞
產品型號: CCL-120.1
產品廠商: 美國標準生物品收藏中心(ATCC)
產品文檔: 無相關文檔


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CCL-120.1 CCRF HSB-2 人急性**母細胞型白血病細胞,原代細胞|細胞系|細胞株|菌種;細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和Z優培養條件!


CCL-120.1 CCRF HSB-2 人急性**母細胞型白血病細胞 的詳細介紹
CCL-120.1 CCRF HSB-2 人急性**母細胞型白血病細胞
ATCC® Number: CCL-120.1?    Price: $338.00
Designations: CCRF-HSB-2 [CCRF HSB-2, HSB-2]
Depositors:  GE Foley
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens (human)
Morphology: lymphoblast

Source: Organ: peripheral blood
Disease: acute lymphoblastic leukemia
Cell Type: T lymphoblast;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Tumorigenic: Yes
Antigen Expression: HLA A1, A2, B12, B17, Cw2; CD5 (78%), CD7 (96%)
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 10,12
D16S539: 9,13
D5S818: 11,12
D7S820: 11,12
THO1: 9,10
TPOX: 8
vWA: 18,19
Isoenzymes: G6PD, B
Age: 11.5 years
Gender: male
Ethnicity: Caucasian
Comments: Derived from the same buffy coat preparation as CCL-120 (CCRF-SB) by serially transplanting into newborn syrian hamsters.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 3 - 5 X 10 exp5 viable cells/ml.
A maximum of 2 - 3 X 10 exp6 viable cells/ml is obtainable.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
recommended serum:ATCC 30-2020
derived from same individual:ATCC CCL-120
References: 24413: . M.D. Anderson Hospital and Tumor Inst. Monograph. 21: ; 1967.
25969: Adams RA, et al. Direct implantation and serial transplantation of human acute lymphoblastic leukemia in hamsters, SB-2. Cancer Res. 28: 1121-1125, 1968. PubMed: 4872716
26035: . . Proc. Am. Assoc. Cancer Res. 8: 1, 1967.
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